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Development of stable cell lines for cell based assay systems

The characterisation of membrane receptors is of particular importance developing inhibitory drugs by the pharmacutical industry. The whole cellular environment is often required for functional receptor activity. Therefore living cells are used stably overexpressing the receptor transgene. We develop fluorescence based assay systems using stable cell lines for your specific requirement.      IIIIII

Basis technology for the development of stable CHO cell lines is our IGAMI expression system. For high throughput screening of potentiell drugs stable cell lines should have a high and stable expression level. Cells that overexpress membrane proteins involved in signal transduction are especially prone to loosing their ability of stably expressing their transgene after a few passages even under selection pressure. Our IGAMI expression technology is particulary suitable for the development of stable cell lines stably expressing such membrane receptor proteins.

Ca2+-influx in CHO-cells stably expressing a recombinant Ca2+-exchanger

Development of suitable vectors The effective transcription and translation of the genetic information in mammalian cells needs optimization of codon-usage and RNA stability. After optimizing we clone your sequences in adequate vectors.	Establishing of recombinant production cell lines For development of production cell lines we use CHO cells growing in chemically defined protein- and peptide-free medium. Our IGAMI expression system allows the fast and easy development of high producer cell clones.
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