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Contract MAB Production

Optimal conditions for cell growth and MAB production are determined on shaker flask level. Appropriate media for the promotion of optimal growth and the best operating parameters for maximum MAB production are determined.

Operation of the bioreactor is closely monitored to ensure adherence to defined production parameters. Cell growth and antibody production are assessed with the periodic measurement of glucose uptake, pH, lactate concentration, antibody yield, temperature, gas exchange, etc.

Contract MAB Purification

With the protein A/G method a mixed subclass IgG preparation can be separated out by eluting a protein A/G column in a single step. This method is highly specific and can be used with almost all isotypes of IgG’s. The purified antibody is then bottled and stored in aliquots in liquid, frozen or lyophilized form depending on the product specifications. Q.C. analysis assessing the purified antibody concentration, purity and functionality is performed.

Development of stable cell lines for cell based assay systems

The characterisation of membrane receptors is of particular importance developing inhibitory drugs by the pharmacutical industry. The whole cellular environment is often required for functional receptor activity. Therefore living cells are used stably overexpressing the receptor transgene. We develop fluorescence based assay systems using stable cell lines for your specific requirement.

    
IIIIII 		Basis technology for the development of stable CHO cell lines is our IGAMI expression system. For high throughput screening of potentiell drugs stable cell lines should have a high and stable expression level. Cells that overexpress membrane proteins involved in signal transduction are especially prone to loosing their ability of stably expressing their transgene after a few passages even under selection pressure. Our IGAMI expression technology is particulary suitable for the development of stable cell lines stably expressing such membrane receptor proteins.


Ca2+-influx in CHO-cells stably expressing a recombinant Ca2+-exchanger

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